Part:BBa_K1073020:Experience

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Applications of BBa_K1073020

The iGEM Team Braunschweig 2013 used this device as a reporter to distinguish two different strains.
When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue.

User Reviews

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••••• iGEM Team Braunschweig 2013

The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absorption by the chomoproteins during OD measurement of a bacterial culture, the absorption and emission spectra were measured. An optimal wavelength for spectral optical density measurement was determined to be at 520nm according to these spectra. iGEM Team Braunschweig 2013: Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm. Absorption maximum of aeBlue: 595 nm iGEM Team Braunschweig 2013:Emission spectrum of the chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence. Emission maximum of aeBlue: none Since the iGEM Team Braunschweig could not detect any emission when prufified aeBlue was excited by UV-light it is possible that this chromoprotein is a so called dark quencher protein. When excited, this kind of chromophores relax back to the ground state without emitting energy through radiation and remain dark [Le Reste et al., 2012]. The optical density of cultures with cells expressing aeBlue was measured at a wavelength of 520 nm to avoid errors in measurement through absorption by the protein.

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